Faezeh Shekari Ph.D.

Department of Stem Cells and Developmental Biology

Assistant professor




Faezeh Shekari is a member of Royan Extracellular Vesicle Group. She graduated in B.Sc and M.Sc of Cellular and Molecular Biology from Tehran University. During her undergraduate studies, she got interested in proteomics. She joined Royan Institute at 2005 to pursue her interests on membrane proteomics. Toward her PhD these, she got interested in subcellular fractionation and organellar proteomics under supervision of Dr Hosseini Salekdeh and Prof. Baharvand. She performed international projects including membrane proteome analysis of liver microsomal fraction from international membrane proteomics initiative (MPI) as well as prepared and characterized standard sample for Asian and Oceania human embryonic stem cell MPI under supervision of Prof. Yu-Ju Chen (from Academia Sinica, Taiwan) and Dr. Hosseini Salekdeh and Prof. Baharvand (from Royan institute) in Academia Sinica. Currently she works as an Assistant Professor in Royan Extracellular Vesicle Group.

Extracellular vesicle/ proteomics and biomarker discovery/ drug delivery/
We are a newly established group with the aim to develop new extracellular vesicle based therapeutics and diagnostic. As a part of Royan Advanced Therapeutic Medicinal Products, we aim to isolate and characterized functional extracellular vesicles to be used in clinical trials. To pursue this goal we try to optimize and select best method of choice whatever ultracentrifugation based methods, size exclusion or filtration and test functionality in vitro and in vivo animal model. Furthermore, we aim to utilize well characterized vesicles as drug delivery vehicle.

Fatemeh Mehryab (PharmD student)

EV-based breast cancer therapy

Afshin Davari (PhD candidate)

Leishmania EV

Abdo-Reza Nazari (MSc)

EV banking

Fatemeh Kabiri (PharmD student)

EV-based breast cancer therapy

Parisa Shamshiri poor (MD student)

EV based biomarker discovery

Selected Publications:

1.     Shekari F, Taei A, Pan TL, Wang PW, Baharvand H, Salekdeh GH. “Identification of cytoplasmic and membrane-associated complexes in human embryonic stem cells using blue native PAGE.” Mol Biosyst.2011 Sep;7(9):2688-701. doi: 10.1039/c1mb05135k. Epub 2011 Jul 5. PubMed PMID: 21727978.

2.      Shekari F, Baharvand H, Salekdeh GH. “Organellar proteomics of embryonic stem cells.” Adv Protein Chem Struct Biol. 2014;95:215-30. doi: 10.1016/B978-0-12-800453-1.00007-5. PubMed PMID: 24985774

3.     Hashemi A, Gharechahi J, Nematzadeh G, Shekari F, Hosseini SA, Salekdeh GH. “Two-dimensional blue native/SDS-PAGE analysis of whole cell lysate protein complexes of rice in response to salt stress.”  J Plant Physiol. 2016 Aug 1;200:90-101. doi: 10.1016/j.jplph.2016.05.023. Epub 2016 Jun 22. PubMed PMID: 27362847.

4.     Shekari F, Nezari H, Larijani MR, Han CL, Baharvand H, Chen YJ, Salekdeh GH. “ Proteome analysis of human embryonic stem cells organelles.” J Proteomics. 2017 Apr 20. pii: S1874-3919(17)30143-4. doi: 10.1016/j.jprot.2017.04.017. PubMed PMID: 28435121

5.     Shekari F, Nezari H, Chen YJ, Baharvand H, Hosseini Salekdeh G. “Data for whole and mitochondrial proteome of human embryonic stem cells.” Data Brief. 2017 Jun 12;13:371-376. doi: 10.1016/j.dib.2017.05.036. eCollection 2017 Aug. PubMed PMID: 28664172

6.     Weldemariam, M.; Han, C.-L.; Shekari, F.; Kitata, R. B.; Chuang, C.-Y.; Hsu, W.-T.; Kuo, H.-C.; Choong, W.-K.; Sung, T.-Y.; He, F.-C.; Chung, M. C. M.; Salekdeh, G. H.; Chen, Y.-J., Subcellular Proteome Landscape of Human Embryonic Stem Cells Revealed Missing Membrane Proteins.” Journal of Proteome Research 2018, Sep 24. doi: 10.1021/acs.jproteome.8b00407.

7.     M Soleymani-Goloujeh, S Saberi, F Shekari . “Extracellular Vesicles in Regenerative Medicine, a Brief Review.” Modern Medical Laboratory Journal, 2018

8.     Shekari F, Han CL, Lee J, Mirzaei M, Gupta V, Haynes PA, lee BH, Baharvand H, Chen YJ, Hosseini Salekdeh G. Surface markers of Human Embryonic Stem Cells: a meta-analysis of membrane proteomics reports” Expert Review of Proteomics, 2018, 2018 Nov;15(11):911-922.

9.     Therry C, Witwer K, et al. “Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines“  Journal of Extracellular Vesicles,  2018, 7:1