Primer Bank
Expert: Monireh Sadat Miri
Introduction
Quantitative Reverse Transcription PCR (qRT-PCR) is a widely used method for analyzing gene expression at the RNA level. This process is carried out using a set of primers, dNTPs, buffers, and enzymes. Among these components, the correct selection of primers is one of the most critical steps in designing a successful qRT-PCR experiment.
Primers are short oligonucleotide sequences, designed and synthesized in various formats, including Oligo dT, Random Hexamers, and Sequence-Specific primers.
Sequence-Specific Primers
Sequence-specific primers are designed to bind to and amplify specific DNA sequences. Therefore, ensuring that primers amplify only the intended sequence and possess the required specificity and sensitivity is crucial. This makes precise primer design an essential element for accurate results.
About the Primer Bank
The Primer Bank of the Molecular Laboratory has been operational since 2007 and provides primers for a wide range of species, including:
- Homo sapiens
- Mus musculus
- Rattus norvegicus
- Canis lupus
- Ovis aries
- Macaca fascicularis
- Chicken
- Oryctolagus cuniculus
Currently, the bank contains over 2,500 primer pairs. This extensive array of primers enables the investigation of the expression of differentiation, pluripotency, oncogenic markers, and other markers across various cell types and species.
Services Provided by the Primer Bank
- Distributing available primers from the bank to students
- Ordering the synthesis of custom-designed primers
- Educational approaches to primer design